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Chapters
- 00:05Introduction
- 00:37Kidney Fixation, Decolorization, and De-Lipidation
- 01:45Whole-Mount Immunofluorescent Staining and Refractive Index (RI) Matching
- 03:03Results: Whole-Mount Immunofluorescent Staining for Three-Dimensional (3D) Kidney Imaging
- 04:00Conclusion
O presente protocolo descreve um método de clareamento tecidual e coloração imunofluorescente de montagem completa para imagens renais tridimensionais (3D). Esta técnica pode oferecer perspectivas macroscópicas na patologia renal, levando a novas descobertas biológicas.
