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基于同步成像和流式细胞术检测治疗诱导的衰老癌细胞中的多种荧光衰老标志物
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Cancer Research
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JoVE Journal
Cancer Research
Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
Please note that all translations are automatically generated.
Click here for the English version.
基于同步成像和流式细胞术检测治疗诱导的衰老癌细胞中的多种荧光衰老标志物
DOI:
10.3791/63973-v
•
08:56 min
•
July 12, 2022
•
Eva Dovjak
,
Mario Mairhofer
,
Claudia Wöß
,
Jingjing Qi
,
Dorothy N. Y. Fan
,
Clemens A. Schmitt
2,3
,
Yong Yu
1
Johannes Kepler University Linz
,
2
Charité – University Medicine Berlin
,
3
Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association
Chapters
00:05
Introduction
00:55
Stain DLBCL Cells with Different Senescence Markers
04:01
Imaging Senescence Markers Using the Imaging Flow Cytometer System
07:12
Results: Visualization and Quantification of Senescent Cells on a Single-Cell Level
08:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一种基于流式细胞术的方法,用于可视化和定量单个细胞中多种衰老相关标记物。
Tags
Simultaneous Imaging
Flow Cytometry
Fluorescent Senescence Markers
Therapy-induced Senescent Cancer Cells
DLBCL Cell Lines
EdU
Chloroquine
C12 FDG
2-phenylethyl-beta-d-thiogalactoside
Paraformaldehyde Fixation
Saponin Permeabilization
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