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Journal
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Cancer Research
/
离体
小鼠尿路上皮细胞腺病毒-Cre介导的基因缺失的类器官模型
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Ex Vivo
Organoid Model of Adenovirus-Cre Mediated Gene Deletions in Mouse Urothelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
离体
小鼠尿路上皮细胞腺病毒-Cre介导的基因缺失的类器官模型
DOI:
10.3791/63855-v
•
09:16 min
•
May 05, 2022
•
Dongbo Xu
,
Li Wang
,
Kyle Wieczorek
,
Yanqing Wang
,
Xiaojing Zhang
,
David W. Goodrich
,
Qiang Li
2
1
Department of Urology
,
Roswell Park Comprehensive Cancer Center
,
2
Department of Pharmacology and Therapeutics
,
Roswell Park Comprehensive Cancer Center
Chapters
00:04
Introduction
00:45
Dissection of Mouse Bladder
02:09
Dissociation of Urothelial Cells
04:41
Counting and Resuspending Cells
05:27
Adenoviral Transduction and Plating Organoids
07:06
Results: Characterization of Triple Knockout Organoids via Ex Vivo Adenovirus-Cre Recombinase
08:31
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
该协议描述了小鼠尿路上皮类器官的产生和表征过程,这些类器官在感兴趣的基因中具有缺失。这些方法包括收获小鼠尿路上皮细胞,用腺病毒用CMV启动子驱动Cre表达的
离体
转导,以及
体外
和
体内
表征。
Tags
AI-generated Organoid Model
Ex Vivo Bladder Cancer Organoids
Cre-mediated Gene Deletion
Mouse Urothelial Cells
Adenovirus Transduction
Cell Dissociation
Collagenase And Hyaluronidase
Centrifugation
Article
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