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Cancer Research
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使用三维类器官培养物离
体
维持人胶质母细胞瘤细胞多样性
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Maintaining Human Glioblastoma Cellular Diversity
Ex vivo
using Three-Dimensional Organoid Culture
Please note that all translations are automatically generated.
Click here for the English version.
使用三维类器官培养物离
体
维持人胶质母细胞瘤细胞多样性
DOI:
10.3791/63745-v
•
07:11 min
•
August 25, 2022
•
Swetha J. Sundar
,
Sajina Shakya
,
Violette Recinos
,
Christopher G. Hubert
1
Department of Neurological Surgery
,
Cleveland Clinic
,
2
Cardiovascular and Metabolic Sciences
,
Lerner Research Institute, Cleveland Clinic
Chapters
00:04
Introduction
01:25
Making Organoid Molds
02:22
Preparing Single‐Cell Suspension from Glioblastoma (GBM) Adherent Culture
03:22
Making Organoids from a Single‐Cell Suspension
05:23
Results: Generation and Maintenance of Human GBM Diversity Ex Vivo from Patient‐Derived Cell Cultures
06:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一种从主要患者标本或患者来源的细胞培养物中生成胶质母细胞瘤(GBM)类器官并将其维持至成熟的方法。这些GBM类器官含有表型不同的细胞群,并在
体外
重建肿瘤微环境。
Tags
Glioblastoma
Organoid Culture
Cellular Diversity
Microenvironment
Drug Efficacy
Resistance
Personalized Medicine
Temperature-sensitive Materials
Single-cell Suspension
Cell Detachment
Neurobasal Medium
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