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Immunology and Infection
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使用真空稳定成像系统的实验性急性肺损伤中肺微循环的活体内宽视场荧光显微镜
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Intravital Widefield Fluorescence Microscopy of Pulmonary Microcirculation in Experimental Acute Lung Injury Using a Vacuum-Stabilized Imaging System
Please note that all translations are automatically generated.
Click here for the English version.
使用真空稳定成像系统的实验性急性肺损伤中肺微循环的活体内宽视场荧光显微镜
DOI:
10.3791/63733-v
•
09:28 min
•
April 06, 2022
•
Stefan Hall
,
Sufyan Faridi
,
Irene Euodia
3
,
Sophie Tanner
,
Andrew Krzysztof Chojnacki
,
Kamala D. Patel
7
,
Juan Zhou
4
,
Christian Lehmann
2,4,8
1
Department of Physiology and Biophysics
,
Dalhousie University
,
2
Department of Microbiology and Immunology
,
Dalhousie University
,
3
Department of Neuroscience
,
Dalhousie University
,
4
Department of Anesthesia, Pain Management, and Perioperative Medicine
,
Dalhousie University
,
5
Live Cell Imaging Center
,
University of Calgary
,
6
Department of Physiology and Pharmacology
,
University of Calgary
,
7
Department of Biochemistry and Molecular Biology
,
University of Calgary
,
8
Department of Pharmacology
,
Dalhousie University
Chapters
00:04
Introduction
00:36
Preparation of the Vacuum-Stabilized Lung Imaging System
01:10
Intubation
01:57
Ventilation
02:47
Thoracotomy
05:10
Microscopy
06:56
Results: Leukocyte Trafficking and Capillary Perfusion in the Pulmonary Microcirculation
08:48
Conclusion
Summary
Automatic Translation
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Automatic Translation
活体荧光显微镜可用于实时研究白细胞 - 内皮相互作用和毛细血管灌注。该协议描述了使用真空稳定的肺成像系统在肺微循环中对这些参数进行成像和量化的方法。
Tags
Intravital Microscopy
Pulmonary Microcirculation
Acute Lung Injury
Vacuum-stabilized Imaging
Leukocyte Adhesion
Capillary Perfusion
Anesthesia
Intubation
Vital Signs Monitoring
Thoracotomy
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