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涙腺幹細胞のための3次元、無血清培養システム
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Biology
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JoVE Journal
Biology
Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
涙腺幹細胞のための3次元、無血清培養システム
DOI:
10.3791/63585-v
•
07:49 min
•
June 02, 2022
•
Hongzi Chen
,
Pan Huang
,
Yan Zhang
1
MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences
,
Sun Yat-sen University
Chapters
00:04
Introduction
00:47
LGSC Primary Culture
03:43
LGSC Maintenance and Passage
04:48
LGSC Differentiation
05:34
Results: Characterization, Differentiation, and Engraftment of LGSCs and Relief of ADDED Symptoms
07:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
成体涙腺(LG)幹細胞の三次元無血清培養法は、LGオルガノイドの形成および腺房または腺管様細胞への分化の誘導のために十分に確立されている。
Tags
Lacrimal Gland
Stem Cells
Three-dimensional
Serum-free
Culture System
Regeneration
Reconstruction
Expansion Capacity
Differentiation
Euthanized Mouse
Dispase
Collagenase I
Trypsin EDTA
Dulbecco’s Modified Eagles Medium
Ham’s F-12
Matrix Gel
Lacrimal Gland Stem Cell Medium
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