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Journal
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Immunology and Infection
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油酸诱导的急性呼吸窘迫综合征小鼠模型
JoVE Journal
Immunology and Infection
Author Produced
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JoVE Journal
Immunology and Infection
Mouse Model of Oleic Acid-Induced Acute Respiratory Distress Syndrome
Please note that all translations are automatically generated.
Click here for the English version.
油酸诱导的急性呼吸窘迫综合征小鼠模型
DOI:
10.3791/63566-v
•
04:45 min
•
June 02, 2022
•
Sarah de Oliveira Rodrigues*
1,2,5
,
Matheus Augusto Patricio de Almeida*
1,2,6
,
Hugo Caire Castro-Faria-Neto
3,4
,
Adriana Ribeiro Silva
3
,
Cassiano Felippe Gonçalves-de-Albuquerque
2,4,5,6
1
Laboratório de Imunofarmacologia
,
Fundação Oswaldo Cruz, FIOCRUZ
,
2
Laboratório de Imunofarmacologia, Departamento de Bioquímica, Instituto Biomédico
,
Universidade Federal do Estado do Rio de Janeiro
,
3
Programa de Pós-Graduação em Biologia Celular e Molecular
,
Instituto Oswaldo Cruz, FIOCRUZ
,
4
Programa de Pós-Graduação em Biologia Molecular e Celular
,
Universidade Federal do Estado do Rio de Janeiro
,
5
Programa de Pós-Graduação em Ciências e Biotecnologia
,
Universidade Federal Fluminense
,
6
Programa de Pós-Graduação em Neurociências
,
Universidade Federal Fluminense
Chapters
00:04
Introduction
01:06
Preparation of Sodium Oleate Solution and Induction of Lung Injury by Oleic Acid
02:15
Bronchoalveolar Lavage Fluid (BALF) Collection
02:59
Results: Lung Injury Mouse Model for Acute Respiratory Distress Syndrome
04:05
Conclusion
Summary
Automatic Translation
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Automatic Translation
本方案描述了使用油酸模拟急性呼吸窘迫综合征(ARDS)的小鼠肺损伤模型。该模型增加了水肿的炎症介质并降低了肺顺应性。油酸以盐形式(油酸盐)使用,因为这种生理形式避免了栓塞的风险。
Tags
Mouse Model
Oleic Acid
Acute Respiratory Distress Syndrome (ARDS)
Alveolar Permeability
Leukocyte Filtration
Intratracheal Administration
Intravenous Administration
Bronchoalveolar Lavage Fluid
Inflammatory Mediators
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