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Immunology and Infection

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小鼠小肠上皮类器官与先天性淋巴细胞的共培养

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Immunology and Infection

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JoVE Journal Immunology and Infection

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Please note that all translations are automatically generated. Click here for the English version.

小鼠小肠上皮类器官与先天性淋巴细胞的共培养

DOI:

10.3791/63554-v

•

08:22 min

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March 23, 2022

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Emily Read*^1,2, Geraldine M. Jowett*^1,2,3, Diana Coman, Joana F. Neves

¹Centre for Host-Microbiome Interactions,King’s College London, ²Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme,King’s College London, ³Present address: Wellcome Trust/Cancer Research UK Gurdon Institute,Cambridge University

Chapters

00:05Introduction
00:52Maintenance of Murine Small Intestinal Organoids
01:55Co-Culture of Small Intestinal Organoids with Innate Lymphoid Cells
05:36Results: Establishing Co-Cultures Between Intestinal Epithelial Cells and ILC1
07:46Conclusion

Summary

Automatic Translation

该协议为建立小鼠小肠类器官，从小鼠小肠固有层中分离1型先天淋巴细胞，以及在两种细胞类型之间建立3维（3D）共培养物以研究肠上皮细胞和1型先天淋巴细胞之间的双向相互作用提供了详细的说明。

Tags

Co-culture Murine Small Intestine Epithelial Organoids Innate Lymphoid Cells In Vitro Model Intestinal Homeostasis Inflammation ILC1 CD44 Gastrointestinal Diseases Thermal Cross-linking Basal Extracellular Matrix Advanced DMEM F12 PBS2 Centrifugation

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