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Journal
/
Biology
/
一种高度可扩展的方法,用于对自发
性Caenorhabditis
线虫进行生态调查
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
A Highly Scalable Approach to Perform Ecological Surveys of Selfing
Caenorhabditis
Nematodes
Please note that all translations are automatically generated.
Click here for the English version.
一种高度可扩展的方法,用于对自发
性Caenorhabditis
线虫进行生态调查
DOI:
10.3791/63486-v
•
09:10 min
•
March 01, 2022
•
Timothy A. Crombie
,
Robyn E. Tanny
,
Claire M. Buchanan
,
Nicole M. Roberto
,
Erik C. Andersen
1
Department of Molecular Biosciences
,
Northwestern University
Chapters
00:04
Introduction
00:34
Collection Preparation
01:21
Field Collection
02:21
Plating Out Field Collections and Isolating Nematodes from Collections
04:06
Exporting S-Plates from Fulcrum
04:41
Check for Proliferation on S-Plates
05:26
Lysis of Isofemale Lines
06:22
PCR of SSU and ITS2 Sequences
07:00
Identifying Nematodes and Processing the Collection Data
08:04
Results: Identifying Nematodes with Sanger Sequencing and Sequence BLAST
08:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议可用于对自生
的Caenorhabditis
线虫进行大规模的生态调查。该方法的主要优点是有效地组织和分析与从自然界收集的线虫相关的生态和分子数据。
Tags
Caenorhabditis Nematodes
Ecological Survey
Mobile Data Collection
Cloud-based Database
Standardized Data Processing
Substrate Temperature
Ambient Temperature
Humidity
OP50 Bacteria
Nematode Isolation
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