JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
蓝藻细胞外囊泡的分离和表征
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolation and Characterization of Cyanobacterial Extracellular Vesicles
Please note that all translations are automatically generated.
Click here for the English version.
蓝藻细胞外囊泡的分离和表征
DOI:
10.3791/63481-v
•
08:44 min
•
February 03, 2022
•
Steven J. Biller*
1
,
María del Carmen Muñoz-Marín*
2
,
Steeve Lima
4,5
,
Jorge Matinha-Cardoso
4
,
Paula Tamagnini
4,6
,
Paulo Oliveira
4,6
1
Department of Biological Sciences
,
Wellesley College
,
2
Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional, Agroalimentario
,
Universidad de Córdoba
,
3
i3S – Instituto de Investigação e Inovação em Saúde
,
Universidade do Porto
,
4
IBMC – Instituto de Biologia Molecular e Celular
,
Universidade do Porto
,
5
MCbiology Doctoral Program, ICBAS – Instituto de Ciências Biomédicas Abel Salazar
,
Universidade do Porto
,
6
Departamento de Biologia, Faculdade de Ciências
,
Universidade do Porto
Chapters
00:05
Introduction
00:38
Concentrating the Vesicle Sample
02:58
Vesicle Isolation and Purification
04:47
Characterization of the Isolated Vesicles Using Transmission Electron Microscopy
05:22
Characterization of the Isolated Vesicles Using Nanoparticle Tracking Analysis
06:38
Results: An Overview of the Cyanobacterial Vesicle Isolation
07:42
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本方案为从蓝藻培养物中分离、浓缩和表征细胞外囊泡提供了详细的描述。还讨论了从不同尺度的培养物中纯化囊泡的方法,方法之间的权衡以及使用现场样品的注意事项。
Tags
Cyanobacteria
Extracellular Vesicles
Purification
Ultrafiltration
Tangential Flow Filtration
Concentration
Environmental Samples
Field Samples
Culture
Protocol
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
对于外小泡的使用流式细胞仪分析技术
小鼠原发性肾管上皮细胞的分离、鉴定及高通量的细胞外流量分析
细胞调理介质细胞外囊泡的流式细胞学分析
卡诺尔哈布炎细胞
外囊泡的纯化与分析
细胞外囊泡的量化和尺寸测定纳米粒子跟踪分析
三维细胞外囊泡的直接随机光学重建显微镜
Scalable Isolation and Purification of Extracellular Vesicles from
Escherichia coli
and Other Bacteria
Isolation of microRNAs from Tick
Ex Vivo
Salivary Gland Cultures and Extracellular Vesicles
从血浆和实体组织中分离和分析可追溯和功能化的细胞外囊泡
培养的人间充质干细胞细胞外囊泡的分离、表征和治疗应用
Read Article