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Cancer Research
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一种用于乳腺肿瘤微环境活体内成像的无标记分割方法
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Cancer Research
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JoVE Journal
Cancer Research
A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment
Please note that all translations are automatically generated.
Click here for the English version.
一种用于乳腺肿瘤微环境活体内成像的无标记分割方法
DOI:
10.3791/63413-v
•
10:39 min
•
May 24, 2022
•
Brian M. Burkel
,
David R. Inman
,
María Virumbrales-Muñoz
2
,
Erica J. Hoffmann
,
Suzanne M. Ponik
3
1
Department of Cell and Regenerative Biology
,
University of Wisconsin-Madison
,
2
Department of Pathology
,
University of Wisconsin-Madison
,
3
Carbone Cancer Center
,
University of Wisconsin-Madison
Chapters
00:04
introduction
00:55
Generation and Surgical Implantation of the Mammary Imaging Window (MIW)
04:13
Positioning and Maintaining Mouse on the Microscope Stage for Imaging
05:52
Set Up for 4D, Intensity-Based, Label-Free Intravital Imaging of Dynamic Cell Behavior
08:02
Fluorescence Lifetime Imaging (FLIM) of NAD(P)H
08:43
Results: Intravital Imaging of the Mammary Tumor Microenvironment
10:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文描述的活体成像方法利用来自代谢辅助因子NAD(P)H的胶原二次谐波和内源性荧光,将未标记的肿瘤微环境非侵入性地分割成肿瘤,基质和血管室,以深入分析4D活体内图像。
Tags
Intravital Imaging
Mammary Tumor Microenvironment
Label-free Segmentation
Collagen Fibers
Autofluorescent Metabolites
Extracellular Matrix
Vascular Structures
Mammary Imaging Window
Surgical Procedure
Mouse
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