00:56Sample Intake, Heat Treatment, and Sample Assignment
01:52Operating Sample Loading Robots
04:09Manual Sample Loading
04:52Addition of Controls to Testing Plates
05:54Performing RT-qPCR and Determining Plate Validity
07:00Interpreting Sample Results
08:08Results: RT-qPCR Detection of N1 (SARS-CoV-2) Synthetic RNA and P1 (Hs_RPP30) Synthetic DNA, Comparison Between Manual and Automated Saliva Transfer N1 Ct Values, and Evaluation of Heat Treatment Methods for Viscosity Reduction in Saliva
The protocol describes a SARS-CoV-2 diagnostic method that utilizes open-source automation to perform RT-qPCR molecular testing of saliva samples. This scalable approach can be applied to clinical public health surveillance as well as to increase the capacity of smaller university laboratories.