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新生儿鼠心在朗根多夫制剂中的应用改良技术

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Medicine

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JoVE Journal Medicine

Modified Technique for the Use of Neonatal Murine Hearts in the Langendorff Preparation

Please note that all translations are automatically generated. Click here for the English version.

新生儿鼠心在朗根多夫制剂中的应用改良技术

DOI:

10.3791/63349-v

•

08:23 min

•

March 04, 2022

•

Matthew B. Barajas, Richard J. Levy

¹Department of Anesthesiology,Vanderbilt University Medical Center, ²Department of Anesthesiology,Columbia University Medical Center

Chapters

00:04Introduction
00:48Fabrication of Aortic Cannula
02:04Organ Harvesting
03:50Cannulation
06:00Functional Measurement
06:59Results: Metabolic and Physiologic Parameters of Isolated Perfused Newborn Murine Hearts
07:53Conclusion

Summary

Automatic Translation

本方案描述了新生儿鼠心脏的体外旋插管和逆行灌注。使用解剖显微镜和钝化的小针头的两人策略允许可靠的插管。纵向收缩张力的量化是使用连接到左心室顶点的力传感器实现的。

Tags

Neonatal Murine Langendorff Preparation Retrograde Perfusion Cardiac Development Cardiomyocyte Isolation Aortic Cannula Krebs-Heneseleit Buffer Heparin Anesthetic Dissection

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