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Cancer Research
/
細胞外フラックス解析を用いた単一3D微細組織スフェロイドのミトコンドリアエネルギー代謝の探索
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis
Please note that all translations are automatically generated.
Click here for the English version.
細胞外フラックス解析を用いた単一3D微細組織スフェロイドのミトコンドリアエネルギー代謝の探索
DOI:
10.3791/63346-v
•
08:15 min
•
February 03, 2022
•
N. J. Coltman
,
G. Rochford
,
N. J. Hodges
,
H. Ali-Boucetta
,
J. P. Barlow
1
School of Biosciences
,
University of Birmingham
,
2
Nanomedicine, Drug Delivery & Nanotoxicology Lab, School of Pharmacy
,
University of Birmingham
,
3
Mitochondrial Profiling Centre
,
University of Birmingham
Chapters
00:05
Introduction
01:03
Probing Mitochondrial Energy Metabolism of Single Spheroids Using XF Technology
02:44
Preparation of XF Assay Medium
03:08
Preparation of the Sensor Cartridge
04:25
Seeding Single Spheroids into the Spheroid Assay Microplate
05:04
Preparation and Loading of Compounds into the Sensor Cartridge for XF Assays
05:31
Results: Mitochondrial Energy Metabolism in 3D Cancer Cell-Line-Derived Spheroids
07:35
Conclusion
Summary
Automatic Translation
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Automatic Translation
これらのプロトコルは、タツノオトシゴの細胞外フラックス分析を使用して、3Dがん細胞株由来スフェロイドにおけるミトコンドリアエネルギー代謝を調べるのに役立ちます。
Tags
Mitochondrial Energy Metabolism
Extracellular Flux Analysis
Cell Lines
Cell Numbers
Phenotypes
Drug Treatments
Drug Discovery
Cancer Research
In Vitro Spheroids
Spheroid Viability
Sensor Cartridge
Calibrant
Assay Kit
Poly-D-Lysine
XF RPMI Medium
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