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小鼠后肢坏死模型中脚垫脉管系统的高分辨率三维成像
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Medicine
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JoVE Journal
Medicine
High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model
Please note that all translations are automatically generated.
Click here for the English version.
小鼠后肢坏死模型中脚垫脉管系统的高分辨率三维成像
DOI:
10.3791/63284-v
•
08:16 min
•
March 16, 2022
•
Antoine J. Ribieras
,
Yulexi Y. Ortiz
2
,
Sophie Shrestha
,
C. Theodore Huerta
,
Hongwei Shao
2
,
Maria E. Boulina
,
Roberto I. Vazquez-Padron
2
,
Zhao-Jun Liu
2
,
Omaida C. Velazquez
2
1
Dewitt Daughtry Family Department of Surgery
,
University of Miami Miller School of Medicine
,
2
Vascular Biology Institute
,
University of Miami Miller School of Medicine
,
3
University of Miami
,
4
Analytical Imaging Core Facility
,
University of Miami
Chapters
00:04
Introduction
01:19
Equipment Setup and DiI Perfusion
04:08
Preparation of Footpad Tissue for Confocal Laser Scanning Microscopy
05:01
Confocal Laser Scanning Microscopy
06:37
Results: Anatomy of the Murine Hindlimb Vasculature
07:20
Conclusion
Summary
Automatic Translation
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Automatic Translation
本方案描述了一种独特的、临床相关的外周动脉疾病模型,该模型将股动脉和静脉电凝与施用一氧化氮合酶抑制剂相结合,以诱导FVB小鼠的后肢坏疽。然后,心内DiI灌注用于脚垫脉管系统的高分辨率三维成像。
Tags
3D Imaging
Foot Pad
Vasculature
Murine Hindlimb
Gangrene Model
DiI Perfusion
Neovascularization
Peripheral Arterial Disease
Critical Limb Ischemia
Preclinical Research
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