Journal
/
Medicine
/
Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae

Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae

DOI:
10.3791/63259-v

•

09:43 min

•

November 22, 2021

•

, , , , ,
1Centre for Discovery Brain Sciences,University of Edinburgh Medical School: Biomedical Sciences, 2Center for Regenerative Therapies at the TU Dresden

Chapters

  • 00:04Introduction
  • 00:51Sample Preparation and the VAST Software Set Up
  • 02:05Set Up ImageJ/Fiji for Laser Lesions and the Python IDE
  • 02:53Performing Laser Lesions on the VAST System
  • 06:29Results: Laser Lesions as an Alternative Tool to Manual Lesions for Studies on Spinal Cord Regeneration
  • 08:50Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes a method to induce tissue-specific and highly reproducible injuries in zebrafish larvae using a laser lesion system combined with an automated microfluidic platform for larvae handling.

Tags

Laser-induced InjuriesSpinal Cord RegenerationZebrafish LarvaeSemi-automated TechniqueVAST EquipmentAnesthetizing LarvaeAutomated Zebrafish ImagingLaser AblationPython IDEScript ManagementPlate HandlingMicroscope FocusingReproducibility

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs

Controlled Cervical Laceration Injury in Mice

A Contusion Model of Severe Spinal Cord Injury in Rats

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

Controlled Cortical Impact Model for Traumatic Brain Injury

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

Calibrated Forceps Model of Spinal Cord Compression Injury

Contrast Enhanced Ultrasound Imaging for Assessment of Spinal Cord Blood Flow in Experimental Spinal Cord Injury

A Neonatal Mouse Spinal Cord Compression Injury Model

Read Article