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전기 생리 학적 연구를위한 성체, 청소년, 애벌레 및 배아 제브라 피쉬에서 심장 및 혈관 평활근 세포 분리
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies
DOI:

07:04 min

February 09, 2022

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Chapters

  • 00:05Introduction
  • 00:42Isolation of Atrium, Ventricle, and Bulbous Arteriosus from Adult, Juvenile, and Larval Zebrafish
  • 01:37Dissociation of Cardiomyocytes from Adult, Juvenile, and Larval Zebrafish for Electrophysiological Studies
  • 02:59Dissociation of Vascular Smooth Muscle Cells from Adult and Juvenile Zebrafish for Electrophysiological Studies
  • 04:14Isolation of Hearts from Embryonic Zebrafish for Electrophysiological Studies
  • 05:21Results: Action Potential Properties from Isolated Zebrafish Cardiomyocytes

Summary

Automatic Translation

본 프로토콜은 전기생리학적 연구에 적합한 성체, 청소년, 유충 및 배아 제브라피쉬(Danio rerio)로부터 생존가능한 심장 및 혈관 평활근 세포의 급성 단리를 기술한다.

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