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Neuroscience
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インタクトマウスおよびゼブラフィッシュ脳における深部組織3光子蛍光顕微鏡
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain
Please note that all translations are automatically generated.
Click here for the English version.
インタクトマウスおよびゼブラフィッシュ脳における深部組織3光子蛍光顕微鏡
DOI:
10.3791/63213-v
•
08:26 min
•
January 13, 2022
•
Yusaku Hontani*
1
,
Najva Akbari*
1
,
Kristine E. Kolkman
,
Chunyan Wu
3
,
Fei Xia
4
,
Kibaek Choe
,
Grace Zhang Wang
,
Mariya Sokolova
,
Joseph R. Fetcho
,
Chris Xu
1
School of Applied and Engineering Physics
,
Cornell University
,
2
Department of Neurobiology and Behavior
,
Cornell University
,
3
College of Veterinary Medicine
,
Cornell University
,
4
Meinig School of Biomedical Engineering
,
Cornell University
Chapters
00:05
Introduction
01:04
Three-Photon Microscopy Setup
02:01
Fish Preparation for Three-Photon Imaging
03:52
Intravital Imaging in Fish
05:50
Intravital Imaging in Mouse
06:39
Results: Three-Photon Imaging in Intact Mouse and Zebrafish Brain with High Spatiotemporal Resolution
07:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
3光子顕微鏡は、マウスやゼブラフィッシュの脳などの生体組織の奥深くで、高い時空間分解能で高コントラストの蛍光イメージングを可能にします。
Tags
Three-photon Microscopy
In Vivo
Mouse Brain
Zebrafish Brain
Neurological Diseases
Alzheimer’s
Autism
Laser Setup
Brewster’s Angle
Agar
Anesthesia
Paralysis
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