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原发性患者特异性主动脉平滑肌细胞的分离和体外半定量实时收缩测量
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JoVE Journal Biology
Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
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原发性患者特异性主动脉平滑肌细胞的分离和体外半定量实时收缩测量

DOI:
10.3791/63122-v

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08:28 min

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February 15, 2022

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, ,
1Department of Vascular Surgery, Amsterdam UMC,Vrije Universiteit Amsterdam, 2Department of Physiology, Amsterdam Cardiovascular Sciences, Amsterdam UMC,Vrije Universiteit Amsterdam, 3Laboratory of Experimental Cardiology, Department of Cardiology,Leiden University Medical Center

Chapters

  • 00:04Introduction
  • 00:45Isolating Primary Human Smooth Muscle Cells (SMCs) from Aortic Biopsies
  • 02:40Isolating Primary Dermal Fibroblasts from Skin Biopsies
  • 04:25Measuring Contraction
  • 06:00Results: Measurement of Aortic SMC Contraction upon Ionomycin Stimulation and SMC Marker Expression
  • 07:51Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了一种基于外植体培养的方法，用于分离和培养原代患者特异性人主动脉平滑肌细胞和真皮成纤维细胞。此外，还提出了一种用于测量细胞收缩和后续分析的新方法，可用于研究这些细胞中患者特异性差异。

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