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使用比率指标对原代神经元中的线粒体谷胱甘肽氧化还原状态进行实时成像
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Neuroscience
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Please note that all translations are automatically generated.
Click here for the English version.
使用比率指标对原代神经元中的线粒体谷胱甘肽氧化还原状态进行实时成像
DOI:
10.3791/63073-v
•
07:47 min
•
October 20, 2021
•
Athanasios Katsalifis
,
Angela Maria Casaril
,
Constanze Depp
,
Carlos Bas-Orth
1
Department of Medical Cell Biology, Institute for Anatomy and Cell Biology
,
Heidelberg University
,
2
Department of Neurogenetics
,
Max-Planck-Institute for Experimental Medicine
Chapters
00:05
Introduction
00:55
Optimization of Scanning Confocal Microscope Settings
01:58
Live Imaging of the Mitochondrial Redox Response to N-methyl-D-aspartate (NMDA) Treatment
03:08
Data Analysis
05:40
Results: Effects of N-methyl-D-aspartate (NMDA) on Neuronal Mitochondria Oxidation
07:04
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了一种方案,用于使用共聚焦活显微镜确定原代海马和皮质神经元中基底氧化还原状态和氧化还原反应的差异。该协议可以应用于其他细胞类型和显微镜,只需最少的修改。
Tags
Mitochondrial Glutathione Redox State
Live Imaging
Primary Neurons
Ratiometric Indicator
Mitochondrial Redox State
Mitochondrial Membrane Potential
Calcium Concentrations
Confocal Microscopy
NMDA
DTT
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