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分析EGFR突变型NSCLC患者衍生类器官靶向治疗的敏感性
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids
Please note that all translations are automatically generated.
Click here for the English version.
分析EGFR突变型NSCLC患者衍生类器官靶向治疗的敏感性
DOI:
10.3791/63039-v
•
08:52 min
•
November 22, 2021
•
Dora Barbosa Rabago
2,3
,
Collin M. Blakely
2
,
Franziska Haderk
2,3
,
Trever G. Bivona
2,3
1
Department of Medicine
,
University of California, San Francisco
,
2
Helen Diller Family Comprehensive Cancer Center
,
University of California, San Francisco
,
3
Department of Cellular and Molecular Pharmacology
,
University of California, San Francisco
Chapters
00:04
Introduction
01:05
Generating Single-Cell Suspension
02:38
Cell Seeding
04:39
Drug Treatment
05:37
Readout by Luminescence-Based Survival Assay
06:52
Results: The Analysis of Treatment Response of EGFR-Mutant NSCLC Organoid Model to Osimertinib
08:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了NSCLC患者衍生类器官模型中靶向信号传导抑制剂药物敏感性的标准化评估。
Tags
Organoids
EGFR-mutant NSCLC
Targeted Therapies
Matrigel
Cell Culture
Dissociation
Trypsinization
Centrifugation
Cell Counting
Cell Suspension
96-well Plate
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