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基于计算机断层扫描的小鼠后肢血管三维可视化的样品制备
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels
Please note that all translations are automatically generated.
Click here for the English version.
基于计算机断层扫描的小鼠后肢血管三维可视化的样品制备
DOI:
10.3791/63009-v
•
04:35 min
•
October 07, 2021
•
Daiki Seya
,
Yuqing Xu
3
,
Toshifumi Mukunoki
,
Kenichi Tsujita
,
Osamu Nakagawa
,
Yuichiro Arima
2,3,4
1
Department of Molecular Physiology
,
National Cerebral and Cardiovascular Center Research Institute
,
2
International Research Center for Medical Sciences (IRCMS)
,
Kumamoto University
,
3
Department of Cardiovascular Medicine, Graduate School of Medical Sciences
,
Kumamoto University
,
4
X-Earth Center, Faculty of Advanced Science and Technology
,
Kumamoto University
Chapters
00:04
Introduction
00:46
Perfusion
02:56
Results: 3D Evaluation of Murine Blood Vessels in The Lower Extremities of the Mouse
03:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了使用微X射线计算机断层扫描的小鼠后肢血管的可视化和定量方法。
Tags
Computed Tomography
3D Visualization
Murine Hind-limb Vessels
Sample Preparation
Heparin
Vasodilation Buffer
Paraformaldehyde
Contrast Medium
Perfusion
Hind-limb Ischemia Model
Femoral Artery
Femoral Vein
Collateral Vessels
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