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高分辨率呼吸测定法,使用基于腔室和板式呼吸计评估细胞和组织中的生物能量学
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Biology
High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers
Please note that all translations are automatically generated.
Click here for the English version.
高分辨率呼吸测定法,使用基于腔室和板式呼吸计评估细胞和组织中的生物能量学
DOI:
10.3791/63000-v
•
09:53 min
•
October 26, 2021
•
Ryan Awadhpersad
,
Christopher B. Jackson
1
Department of Biochemistry and Developmental Biology, Faculty of Medicine
,
University of Helsinki
Chapters
00:04
Introduction
01:01
High-Resolution Respirometry: Chamber-Based Respirometer (cHRR)
04:51
High-Resolution Respirometry: Microplate-Based Respirometer (mHRR)
06:48
Results: Oxygen Consumption Traces from cHRR and mHRR Using HEK293 Cells with Combined OXPHOS Deficiency
08:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
使用高分辨率呼吸计评估氧化磷酸化已成为线粒体和细胞能量代谢功能分析的一个组成部分。在这里,我们提出了使用基于腔室和微孔板的高分辨率呼吸计分析细胞能量代谢的方案,并讨论了每种设备的主要优点。
Tags
High-resolution Respirometry
Bioenergetics
Mitochondrial Dysfunction
Oxidative Phosphorylation
HEK293 Cells
OXPHOS Analysis
Oligomycin
FCCP
Rotenone
Antimycin
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