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배양된 뼈 세포와 분리된 뼈축의 아미노산 소비 평가
JoVE Journal
Medicine
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JoVE Journal
Medicine
Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts
Please note that all translations are automatically generated.
Click here for the English version.
배양된 뼈 세포와 분리된 뼈축의 아미노산 소비 평가
DOI:
10.3791/62995-v
•
06:32 min
•
April 13, 2022
•
Leyao Shen
,
Courtney M. Karner
2
1
Department of Internal Medicine
,
University of Texas Southwestern Medical Center
,
2
Charles and Jane Pak Center for Mineral Metabolism and Clinical Research
,
University of Texas Southwestern Medical Center
Chapters
00:04
Introduction
00:57
Amino Acid Uptake in Cells
03:02
Amino Acid Uptake in Freshly Isolated Bone Tissues
04:32
Results: L-[3,4-
3
H]-Glutamine Uptake in In Vitro and L-[2,3,4-
3
H]-Arginine Uptake Ex Vivo
05:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
이 프로토콜은 방사성 표지 된 아미노산 섭취 분석을 제시하며, 이는 일차 세포 또는 분리 된 뼈에서 아미노산 소비를 평가하는 데 유용합니다.
Tags
Amino Acid Uptake
Bone Cells
Bone Shafts
Radiolabeled Amino Acids
Osteoblast
Cell Culture
ST2 Cells
Krebs-Ringer’s HEPES
Tritiated Glutamine
Sodium Dodecyl Sulfate
Scintillation Counter
Cell Counting
Bone Marrow
Decellularization
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