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Journal
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Immunology and Infection
/
流式细胞术分析鉴定鼠肺先天免疫细胞和适应性免疫细胞
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
Please note that all translations are automatically generated.
Click here for the English version.
流式细胞术分析鉴定鼠肺先天免疫细胞和适应性免疫细胞
DOI:
10.3791/62985-v
•
09:57 min
•
November 16, 2021
•
Anthos Christofides
2
,
Carol Cao
2,3
,
Rinku Pal
2
,
Halil I. Aksoylar
2
,
Vassiliki A. Boussiotis
2
1
Division of Hematology-Oncology
,
Beth Israel Deaconess Medical Center, Harvard Medical School
,
2
Department of Medicine
,
Beth Israel Deaconess Medical Center, Harvard Medical School
,
3
Harvard College
Chapters
00:04
Introduction
01:17
Surgical Excision and Tissue Preparation
02:11
Preparation of Single-Cell Suspension
04:12
Surface Antibody Staining
05:25
Cell Fixation and Intracellular Staining
06:46
Results: Gating Strategy
09:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这项研究中,我们提出了一种有效且可重复的方案来分离小鼠呼吸系统的免疫群体。我们还提供了一种方法,使用基于9色的流式细胞术面板来鉴定驻留在健康小鼠肺部的所有先天性和适应性免疫细胞。
Tags
Lung Immune Cells
Myeloid Cells
Non-myeloid Cells
Flow Cytometry
Gating Strategy
Lung Immune Landscape
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