Journal
/
Immunology and Infection
/
实时监测细胞因子分化人原代T细胞中的线粒体呼吸
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
Please note that all translations are automatically generated. Click here for the English version.

实时监测细胞因子分化人原代T细胞中的线粒体呼吸

DOI:
10.3791/62984-v

•

06:55 min

•

October 19, 2021

•

, , , , , ,
1National Center for Cancer Immune Therapy, Department of Oncology,University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging,University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group,University of Copenhagen

Chapters

  • 00:05Introduction
  • 00:39Activating Human Primary T Lymphocytes
  • 01:55Extracellular Flux Assay
  • 04:52Results: Optimizing Mitochondrial Modulator Concentrations and Cell Numbers for Monitoring Mitochondrial Respiration in Human Primary T Cells
  • 06:26Conclusion

Summary

Automatic Translation

Automatic Translation

代谢适应是 T 细胞的基础，因为它决定了分化、持久性和细胞毒性。本文介绍了一种用于监测 离体 细胞因子分化的人原代T细胞中线粒体呼吸的优化方案。

Tags

Mitochondrial RespirationT CellCytokineSeahorse MachineExtracellular Flux AssayCell-TakXF Cell Culture PlateAssay MediaGlucosePyruvateGlutamine

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

T细胞受体在小鼠T细胞的逆转录病毒转导

从初级人类的幼稚和记忆T细胞的诱导调节性T细胞的生成

piggyBac转座子初级人类T细胞系统改造

T细胞实时成像实时信号复合物的形成

一种优化的协议分析和糖酵解线粒体呼吸的淋巴细胞

人外周血和淋巴组织中原发性 T 细胞突触界面的评价

人正常记忆 b 细胞与长寿浆细胞体外分化模型

用于嵌合抗原受体 T 细胞的实时效力测定，用于定位固体和血液癌细胞

恒流中原发性人类内皮细胞的肺炎球菌感染

使用实时细胞分析监测宿主外的流感病毒存活率

Read Article