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Neuroscience
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从新生小鼠中磁分离小胶质细胞用于原代细胞培养
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures
Please note that all translations are automatically generated.
Click here for the English version.
从新生小鼠中磁分离小胶质细胞用于原代细胞培养
DOI:
10.3791/62964-v
•
07:23 min
•
July 25, 2022
•
Cindy Bokobza*
1
,
Alice Jacquens*
1,2
,
Manuela Zinni
,
Valérie Faivre
,
Jennifer Hua
,
David Guenoun
,
Caroline Userovici
,
Shyamala Mani
,
Vincent Degos
2
,
Pierre Gressens
,
Juliette Van Steenwinckel
1
NeuroDiderot, Inserm UMR-1141, Hôpital Robert Debré 48
,
Université de Paris
,
2
Department of anesthesia and critical care
,
APHP-Sorbonne university
Chapters
00:05
Introduction
00:32
Brain Dissection
01:29
Brain Dissociation and Magnetic Microglial Isolation
04:23
Phagocytic Assay
05:03
Results: Phagocytic Activity and Magnetic Isolation of Microglia In Vitro
06:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
原代小胶质细胞培养通常用于评估新的抗炎分子。本协议描述了一种可重复且相关的方法,用于从新生儿幼崽中磁性分离小胶质细胞。
Tags
Microglia
Magnetic Cell Sorting
Neonate Mouse
Primary Cell Culture
CD11b
Cell Dissociation
HBSS
Sorting Buffer
Cell Separation
Cell Count
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