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Journal
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Immunology and Infection
/
单纯疱疹病毒的扁平化
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Plaquing of Herpes Simplex Viruses
Please note that all translations are automatically generated.
Click here for the English version.
单纯疱疹病毒的扁平化
DOI:
10.3791/62962-v
•
04:41 min
•
November 05, 2021
•
Lauren A. Sadowski*
1
,
Gregory M. Lesko*
1
,
Chad Suissa*
1
,
Rista Upadhyay*
1,2
,
Prashant J. Desai
,
Barry J. Margulies
4
1
Towson University Herpes Virus Lab, Department of Biological Sciences
,
Towson University
,
2
Towson University Department of Chemistry
,
Towson University
,
3
Department of Oncology
,
Johns Hopkins University School of Medicine
,
4
Molecular Biology, Biochemistry, and Bioinformatics Program
,
Towson University
Chapters
00:04
Introduction
00:53
Sample Dilution
01:18
Addition of Virus to the Cells
02:25
Staining for Plaques
03:04
Results: Effect of Dilution Factor on Plaque Count
03:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
Plaquing是一种用于量化人群中活病毒的常规方法。虽然在各种微生物学课程中经常教授细菌和噬菌体的诱捕,但哺乳动物病毒的幽射更为复杂和耗时。该协议描述了可靠地用于单纯疱疹病毒常规工作的程序。
Tags
Plaquing
Herpes Simplex Virus
Virus Titer Quantification
Antiviral Treatments
Cell Seeding
Virus Dilution
Virus Adsorption
Methylcellulose Overlay
Crystal Violet Staining
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