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Journal
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Cancer Research
/
乳腺上皮和内皮细胞球状体作为乳腺癌研究的潜在功能
体外
模型
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Cancer Research
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JoVE Journal
Cancer Research
Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional
In vitro
Model for Breast Cancer Research
Please note that all translations are automatically generated.
Click here for the English version.
乳腺上皮和内皮细胞球状体作为乳腺癌研究的潜在功能
体外
模型
DOI:
10.3791/62940-v
•
08:30 min
•
July 12, 2021
•
Giovanna Azzarito
,
Marta Ewa Szutkowska
,
Annalisa Saltari
,
Edwin K. Jackson
,
Brigitte Leeners
,
Marinella Rosselli
,
Raghvendra K. Dubey
3
1
Department of Reproductive Endocrinology, Wagistrasse 14
,
University of Zurich and University Hospital Zurich
,
2
Department of Dermatology, Wagistrasse 18
,
University of Zurich and University Hospital Zurich
,
3
Department of Pharmacology and Chemical Biology
,
University of Pittsburgh
Chapters
00:04
Introduction
00:49
Spheroid Formation
03:55
Spheroid Immunohistochemistry Study
05:24
Live/Dead Staining in Spheroids
05:56
Results: Generation of Mammary Epithelial and Endothelial Cell Spheroids
07:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
乳腺上皮细胞和内皮细胞之间的串扰对乳腺癌进展、肿瘤生长和转移有重要贡献。在这项研究中,球状体是由乳腺癌细胞与血管和/或淋巴内皮细胞一起制成的,并证明了它们作为乳腺癌研究
的体外
系统的适用性。
Tags
Breast Cancer
Cell Spheroids
3D Model
Mammary Epithelial Cells
Endothelial Cells
Cancer Cell-endothelial Cell Interactions
Proliferation
Metastasis
In Vitro Model
Cell Distribution
Dye Staining
Enzymatic Detachment
Cell Counting
Cell Suspension
Cell Co-culture
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