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拟南芥种子表面灭菌的高通量、稳健性和高时间灵活性方法
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JoVE Journal Biology
High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds
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拟南芥种子表面灭菌的高通量、稳健性和高时间灵活性方法

DOI:
10.3791/62893-v

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07:28 min

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October 04, 2021

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, , ,
1Department of Biodiversity and Molecular Ecology, Research and Innovation Centre,Fondazione Edmund Mach

Chapters

  • 00:04Introduction
  • 00:46Reagents and Media Preparation
  • 01:59Aspirator Setup
  • 02:37High-Throughput Liquid Surface Sterilization of Seeds
  • 04:56Plating and Scoring of Arabidopsis on Half Strength Murashige and Skoog Plates
  • 05:32Results: Germination Analysis of Arabidopsis Seeds After Surface Sterilization with the New Method
  • 06:57Conclusion

Summary

Automatic Translation

Automatic Translation

提供用于 拟南芥（Arabidopsis） 种子表面灭菌的高通量方案，通过带有真空泵的简单抽吸装置优化液体处理步骤。数百个种子样品可以在一天内进行表面灭菌。

Tags

ArabidopsisSeed Surface SterilizationHigh-throughputRobustTime-flexibleFunctional GenomicsModel Plant SpeciesEthanolBleachMurashige And Skoog MediumAgarKanamycinAspiratorVacuum PumpDecantation BottlePolyethylene TubeAquarium Valve

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