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Journal
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Cancer Research
/
一种三维球体模型研究肝细胞癌肿瘤-基质相互作用
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma
Please note that all translations are automatically generated.
Click here for the English version.
一种三维球体模型研究肝细胞癌肿瘤-基质相互作用
DOI:
10.3791/62868-v
•
12:24 min
•
September 30, 2021
•
Marco Y. W. Zaki*
1,2
,
Shishir Shetty*
2
,
Alex L. Wilkinson
,
Daniel A. Patten
,
Fiona Oakley*
3
,
Helen Reeves*
4,5
1
Department of Biochemistry, Faculty of Pharmacy
,
Minia University
,
2
National Institute for Health Research Birmingham Liver Biomedical Research Unit and Centre for Liver and Gastrointestinal Research, Institute of Immunology and Immunotherapy
,
University of Birmingham
,
3
Newcastle Fibrosis Research Group, Biosciences Institute, Faculty of Medical Sciences
,
Newcastle University
,
4
Newcastle University Translational and Clinical Research Institute, Faculty of Medical Sciences
,
Newcastle University
,
5
The Liver Unit, Department of Medicine, Freeman Hospital
,
Newcastle-upon-Tyne Hospitals NHS Foundation Trust
Chapters
00:05
Introduction
01:20
Cell Preparation
02:17
Cell Collection and Counting
04:14
Collection of Fibroblast Conditioned Media
05:17
Heterotypic Tumor/Stromal Spheroids
06:29
Homotypic Hep3B Spheroids in LX2 Conditioned Media
08:18
Calculation of Spheroid Volume
09:17
Results: In Vitro Creation and Culture of 3D Tumor Spheroid Model
11:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
缺乏能够忠实地概括相关人类疾病的综合
体外
模型。目前的研究提出了三维(3D)肿瘤球体的创建和培养,这是一种可靠的
体外
工具,用于研究人肝细胞癌中的肿瘤 - 基质相互作用。
Tags
3D Tumor Spheroids
Tumor-stromal Interaction
Hepatocellular Carcinoma
Hanging Droplets Method
3D Tumoroids
Drug Screening
Huh7
Hep3B
COS-7
LX2 Fibroblast
Cell Culture
Cell Counting
Trypan Blue
Article
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