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三维细胞外囊泡的直接随机光学重建显微镜
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JoVE Journal Biology
Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
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三维细胞外囊泡的直接随机光学重建显微镜

DOI:
10.3791/62845-v

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09:36 min

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August 26, 2021

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, ,
1Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center,The University of North Carolina at Chapel Hill School of Medicine

Chapters

  • 00:04Introduction
  • 00:56Fixation and Preparation
  • 02:21Direct Stochastic Optical Reconstruction Microscopy Calibration
  • 04:56Visualization of EV in Three Dimensions
  • 06:16Post-Acquisition Modification and EV Tracing
  • 07:13Results: Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

直接随机光学重建显微镜（dSTORM）用于绕过光学显微镜的典型衍射极限，并在纳米尺度上观察外泌体。它可以在二维和三维空间中用于表征外泌体。

Tags

Extracellular VesiclesDSTORMSuper-resolution MicroscopyThree-dimensional VisualizationVirus ParticlesDisease BiomarkersAffinity PurificationParaformaldehyde FixationImaging BufferCalibration Beads

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