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Immunology and Infection
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使用双氟比度显微镜测量巨无素体的pH值、重度化学和降解能力
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Immunology and Infection
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Immunology and Infection
Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用双氟比度显微镜测量巨无素体的pH值、重度化学和降解能力
DOI:
10.3791/62733-v
•
07:31 min
•
August 19, 2021
•
Liam Wilkinson
2
,
Johnathan Canton
3
1
Department of Biochemistry & Microbiology, Faculty of Science
,
University of Victoria
,
2
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Science
,
University of Calgary
,
3
Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases
,
University of Calgary
Chapters
00:05
Introduction
00:28
Measuring Macropinosome pH
02:06
In Situ Calibration of Macropinosome pH
03:04
Measuring Oxidative Events within Macropinosomes
04:22
Measuring Protein Digestion within Macropinosomes
05:30
Results: Measuring the pH, Oxidative Events, and Protein Degradation in Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
06:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了测量活细胞中单个巨细胞体中的pH值、氧化事件和蛋白质消化的方案。强调双氟比显微镜及其提供的优势,而不是基于人群的技术。
Tags
Macropinosomes
PH
Redox
Degradation
Dual-fluorophore
Ratiometric Microscopy
Fluorescein
TMR
HBSS
Potassium Rich Solution
Nigericin
RAW264.7 Cells
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