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Immunology and Infection
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人性化调解员发布分析作为过敏原效力的读出
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
Please note that all translations are automatically generated.
Click here for the English version.
人性化调解员发布分析作为过敏原效力的读出
DOI:
10.3791/62702-v
•
10:22 min
•
June 29, 2021
•
Mario Wenger
,
Athanasios Bethanis
,
Litty Johnson
,
Lorenz Aglas
1
Department of Biosciences
,
University of Salzburg
Chapters
00:04
Introduction
01:04
Complement Inactivation of Human Sera
02:05
Humanized-RBL Cell Culture
04:07
Passive Sensitization of Humanized-RBL Cells
04:57
Antigen-stimulated Degranulation and Mediator Release
06:19
β-hexosaminidase Activity Measurement
07:21
Results: Effect of Allergen on β-hexosaminidase Release
09:38
Conclusion
Summary
Automatic Translation
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在这里,我们介绍介质释放分析,使用与人类IgE受体相互感染的鼠血友病白血病细胞系,模拟在1型过敏反应中通常观察到的效应细胞的去粒。该方法以高度敏感、可重复和可定制的方式研究过敏原的生物活性。
Tags
Humanized Mediator Release Assay
Allergen Potency
Type 1 Allergic Reactions
Recombinant Allergen
Purified Allergen
Allergen Extract
IgE Reactivity
Cross-reactivity
Allergen Immunotherapy (AIT) Treatment Efficacy
Ag8 Cells
Humanized RBL Cells
Cell Culture
Cell Suspension
Cell Counting
96-well Plate
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