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Journal
/
Cancer Research
/
ポリ無水物IL-1αナノ粒子の
生体内
抗腫瘍活性の評価
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Evaluation of the
In vivo
Antitumor Activity of Polyanhydride IL-1α Nanoparticles
Please note that all translations are automatically generated.
Click here for the English version.
ポリ無水物IL-1αナノ粒子の
生体内
抗腫瘍活性の評価
DOI:
10.3791/62683-v
•
09:57 min
•
June 28, 2021
•
M. M. Hasibuzzaman
2
,
Kathleen A. Ross
4
,
Aliasger K. Salem
4,5,6
,
Balaji Narasimhan
4
,
Andrean L. Simons
2,3,5,6,7
1
Interdisciplinary Graduate Program in Human Toxicology
,
University of Iowa
,
2
Department of Pathology
,
University of Iowa
,
3
Department of Chemical and Biological Engineering, College of Engineering
,
Iowa State University
,
4
Nanovaccine Institute
,
Iowa State University
,
5
Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy
,
University of Iowa
,
6
Holden Comprehensive Cancer Center
,
University of Iowa
,
7
Department of Oral Pathology, Radiology and Medicine, College of Dentistry
,
University of Iowa
Chapters
00:04
Introduction
00:55
Preparation and Maintenance of HNSCC Cell Line
02:38
Tumor Implantation
03:14
Blood Collection and Serum Separation
04:20
Multiplexing of Collected Serum
04:51
Collection of Tumor and Inguinal Lymph Node and Preparation of Single-Cell Suspension
05:58
FACS Staining of Single-Cell Suspension
07:42
Results: IL-1α Antitumor Activity in HNSCC Mouse Model
09:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
HNSCCの同系マウスモデルにおけるIL-1αの抗腫瘍活性および関連する毒性を研究するための標準プロトコルが記載されている。
Tags
In Vivo Antitumor Activity
Polyanhydride IL-1α Nanoparticles
Tumor Implantation
Cytokine Analysis
Immune Cell Populations
Antitumor Efficacy
Toxicity Studies
EERL Cells
Cell Culture
Subcutaneous Injection
Submandibular Blood Collection
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