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确定紫外线辐射和化学物质对原代和永生化的人角膜上皮细胞的毒性
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Biology
Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
确定紫外线辐射和化学物质对原代和永生化的人角膜上皮细胞的毒性
DOI:
10.3791/62675-v
•
09:31 min
•
July 22, 2021
•
Jaclyn M. L. Chang
,
Junghee Seo
,
Maggie Miu Yee Kwan
,
Sarah Oh
,
David J. McCanna
,
Lakshman Subbaraman
,
Lyndon Jones
2
1
Centre for Ocular Research & Education (CORE), School of Optometry & Vision Science
,
University of Waterloo
,
2
Centre for Eye and Vision Research (CEVR)
Chapters
00:05
Introduction
00:56
Determination of Cell Size Using Confocal Microscopy
01:59
Exposure of Cells to Ultraviolet (UV) Radiation
03:50
Exposure of Cells to Chemical Toxins
05:31
Examination of Cells Exposed to Various Concentrations of BAK
06:53
Results: Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
08:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了用于评估紫外线辐射和化学毒素对原代和永生化细胞系的毒性的程序。
Tags
Toxicity
UV Radiation
Chemicals
Primary Human Corneal Epithelial Cells
Immortalized Human Corneal Epithelial Cells
In Vitro Assay
Cell Viability
Annexin Staining
Confocal Microscopy
Cytokine Release
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