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Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
This content is Free Access.
Chapters
- 00:04Introduction
- 00:55Mechanical and Enzymatic Digestion of the Uterus
- 02:35Processing of the Uterus into a Single Cell Suspension
- 05:22Red Blood Cell Lysis
- 06:13Results: FACS Analysis of Innate Lymphoid Cells (ILCs)
- 08:06Conclusion
This is a method to isolate uterine lymphoid cells from both pregnant and non-pregnant mice. This method can be used for multiple downstream applications such as FACS phenotyping, cell sorting, functional assays, RNA-seq, and proteomics. The protocol here demonstrates how to phenotype group 1 uterine innate lymphoid cells by flow cytometry.
