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Journal
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Immunology and Infection
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从人类胚胎干细胞中引导肝细胞样细胞诱导的有效方法
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Immunology and Infection
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JoVE Journal
Immunology and Infection
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
从人类胚胎干细胞中引导肝细胞样细胞诱导的有效方法
DOI:
10.3791/62654-v
•
08:05 min
•
May 06, 2021
•
Qi Zhou*
1,2,3
,
Xiaoling Xie*
1,2,3
,
Zhiqian Zhong
2,3
,
Pingnan Sun
2,3
,
Xiaoling Zhou
2,3
1
Stem Cell Research Center
,
Shantou University Medical College
,
2
The Center for Reproductive Medicine
,
Shantou University Medical College
,
3
Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology
,
Shantou University Medical College
Chapters
00:04
Introduction
00:45
Stem Cell Maintenance
02:28
Stem Cell Passage and Differentiation and Definitive Endoderm Formation
03:46
Differentiation of Hepatic Progenitor Cells and Hepatocyte Differentiation
05:12
Results: Directed Hepatocyte-like Cell Induction from Human Embryonic Stem Cells
07:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本手稿描述了人类胚胎干细胞(hESC)分化成功能性肝细胞样细胞(HLCs)的详细协议,在hESC分化过程中连续补充Activin A和CHIR99021,以最终内分泌体(DE)。
Tags
Human Embryonic Stem Cells
Hepatocyte-like Cells
Differentiation
Definitive Endoderm
FOXA2
SOX17
GATA4
CXCR4
FOXA1
Matrigel
MTESR
Activin A
CHIR99021
Cell Culture
Disease Modeling
Drug Screening
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