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逆行帕罗蒂德格兰德输液通过斯滕森的管道在非人类灵长类病原体矢量基因交付
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Immunology and Infection
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JoVE Journal Immunology and Infection
Retrograde Parotid Gland Infusion through Stensen’s Duct in a Non-Human Primate for Vectored Gene Delivery
Please note that all translations are automatically generated. Click here for the English version.

逆行帕罗蒂德格兰德输液通过斯滕森的管道在非人类灵长类病原体矢量基因交付

DOI:
10.3791/62645-v

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03:50 min

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August 12, 2021

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, , , , ,
1Department of Medicine, Division of Infectious Diseases and Global Medicine,University of Florida, 2Department of Otolaryngology,George Washington University School of Medicine, 3Department of Medicine, Section on Infectious Diseases,Wake Forest Baptist Medical Center, 4Department of Pathology,Wake Forest School of Medicine, 5Department of Medicine, Section of Infectious Diseases,Hefner Veterans Affairs Medical Center

Chapters

  • 00:04Introduction
  • 00:49Performing the Procedure
  • 02:49Results: Successful Transduction Following Retrograde Parotid Gland Infusion of Ad5-EGFP in a Non-Human Primate
  • 03:22Conclusion

Summary

Automatic Translation

Automatic Translation

唾液腺已被提议为基因治疗的组织靶点，特别是在通过基因转移接种疫苗方面。我们演示了利用逆行性皮质输液的非人类灵长类动物模型中的基因传递。

Tags

Retrograde Parotid Gland InfusionStensen’s DuctNon-human PrimateVectored Gene DeliveryParotid PapillaPolyethylene TubeCyanoacrylateFluoroscopyEGFPGene TherapySalivary Gland

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