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使用生物发光成像对小鼠尿路感染及其治疗的纵向随访
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Immunology and Infection
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JoVE Journal Immunology and Infection
Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging
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使用生物发光成像对小鼠尿路感染及其治疗的纵向随访

DOI:
10.3791/62614-v

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07:39 min

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June 14, 2021

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, , , , , , ,
1Laboratory of Ion Channel Research (LICR), VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium & Department of Cellular and Molecular Medicine,KU Leuven, 2Biomedical MRI, Department of Imaging & Pathology,KU Leuven, 3Belgium & KU Leuven Centre of Microbial and Plant Genetics,VIB-KU Leuven Center for Microbiology, Leuven, 4Laboratory of Experimental Urology, Department of Development and Regeneration,KU Leuven

Chapters

  • 00:04Introduction
  • 00:57Culturing Bacteria
  • 02:36Inoculation of the Animals
  • 04:04Bioluminescence Imaging
  • 05:49Results: The Analysis of BLI Correlation with CFU at the Time of Instillation and Longitudinal Follow-up During Treatment
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

本手稿描述了使用 勒克斯 操纵子膀胱内施用泌尿道致病细菌以诱导小鼠尿路感染，以及随后使用生物发光成像对细菌负荷进行 纵向体内 分析。

Tags

Bioluminescence ImagingUrinary Tract InfectionsUTILongitudinal Follow-upBacterial CultureInoculum PreparationAnesthesiaIntravesical Instillation

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