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Journal
/
Neuroscience
/
利用
体内
出生后电穿孔研究小脑颗粒神经元形态和突触发育
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Utilizing
In Vivo
Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development
Please note that all translations are automatically generated.
Click here for the English version.
利用
体内
出生后电穿孔研究小脑颗粒神经元形态和突触发育
DOI:
10.3791/62568-v
•
04:20 min
•
June 09, 2021
•
Urann Chan
,
Diwas Gautam
,
Anne E. West
1
Department of Neurobiology
,
Duke University Medical Center
Chapters
00:05
Introduction
00:39
Preparing the Injection Syringe for Electroporation
01:00
Morphological Analyses of Cerebellar Granule Neurons (CGNs): 3D Reconstruction
02:34
Results: Analysis of Granule Neuron Morphology and Size During Cerebellar Development
03:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一种方法,当这些细胞细化其突触结构并形成突触以整合到整个脑回路中时,在出生后大脑发育的时间过程中可视化小鼠小脑中颗粒神经元的突触发生。
Tags
Cerebellar Granule Neurons
In Vivo Postnatal Electroporation
Neurite Morphology
Dendritic Claw Formation
Confocal Microscopy
Simple Neurite Tracer
Fiji
3D Reconstruction
Developmental Processes
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