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使用小鼠胚胎干细胞进行血管疾病建模和药物测试的血管生成的三维发芽测定
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In Vitro
Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
Please note that all translations are automatically generated.
Click here for the English version.
体外
使用小鼠胚胎干细胞进行血管疾病建模和药物测试的血管生成的三维发芽测定
DOI:
10.3791/62554-v
•
08:04 min
•
May 11, 2021
•
Georgios Galaris
,
Jérémy H. Thalgott
,
Eliott Teston
,
Franck P.G. Lebrin
2,3
1
Einthoven Laboratory for Experimental Vascular Medicine, Department of Internal Medicine (Nephrology)
,
Leiden University Medical Center
,
2
Institute Physics for Medicine Paris
,
INSERM U1273, ESPCI Paris, CNRS FRE 2031
,
3
MEMOLIFE Laboratory of Excellence and PSL Research University
Chapters
00:04
Introduction
01:14
Embryoid Bodies (EB) Formation in Hanging Drop
03:02
Floating EBs Culture for Vascular Differentiation
03:41
3D Sprouting Angiogenesis Assay and Immunofluorescence Staining
05:44
Results: Microscopic Analysis and Quantification of Mouse EBs Undergoing Angiogenesis Assay
07:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
该测定利用分化成在3D胶原蛋白凝胶中培养的拟胚体的小鼠胚胎干细胞来分析体
外
控制发芽血管生成的生物学过程。该技术可用于测试药物,模拟疾病以及在胚胎致命的缺失背景下研究特定基因。
Tags
In Vitro
Sprouting Angiogenesis
Mouse Embryonic Stem Cells
Vascular Disease Modeling
Drug Testing
Endothelial Tip Cells
Cell Migration
Genetic Screening
Pluripotency
Karyotyping
Mesoderm Differentiation
Low Attachment Polystyrene Dishes
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