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Cancer Research
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将原发性癌症相关纤维细胞与乳腺癌的合成素 Murine 模型分离,用于靶向纳米粒子的研究
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles
Please note that all translations are automatically generated.
Click here for the English version.
将原发性癌症相关纤维细胞与乳腺癌的合成素 Murine 模型分离,用于靶向纳米粒子的研究
DOI:
10.3791/62504-v
•
08:02 min
•
May 14, 2021
•
Marta Truffi*
1
,
Leopoldo Sitia*
2
,
Marta Sevieri
,
Arianna Bonizzi
,
Maria Antonietta Rizzuto
,
Serena Mazzucchelli
,
Fabio Corsi
2
1
Istituti Clinici Scientifici Maugeri IRCCS
,
2
Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”
,
Università di Milano
,
3
Dipartimento di Biotecnologie e Bioscienze
,
Università di Milano-Bicocca
Chapters
00:04
Introduction
00:37
Tumor Dissociation into Single Cells
02:55
Extraction of Cancer-Associated Fibroblasts (CAFs) from Dissociated Tumor
05:20
Results-I: Isolation and Culture of Primary CAFs from a Syngeneic Murine Breast Cancer
06:40
Results-II: Use of Isolated CAFs to Evaluate Targeting Potential of Hfn-FAP Functionalized Nanodrugs
07:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文旨在为三阴性乳腺癌的同步粘膜模型中原发性癌症相关成纤维细胞的分离与培养提供协议,并应用于旨在针对肿瘤微环境的新型纳米粒子的临床前研究。
Tags
Primary Cancer-associated Fibroblasts
Murine Breast Cancer
Tumor Microenvironment
In Vitro Studies
Tumor Stroma
Tumor Dissociation
Cell Isolation
Dead Cell Removal
Magnetic Separation
Cell Culture
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