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小鼠新鲜骨髓外植体的丙酸盐形成动力学
JoVE Journal
Biology
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JoVE Journal
Biology
Proplatelet Formation Dynamics of Mouse Fresh Bone Marrow Explants
Please note that all translations are automatically generated.
Click here for the English version.
小鼠新鲜骨髓外植体的丙酸盐形成动力学
DOI:
10.3791/62501-v
•
05:58 min
•
May 20, 2021
•
Inès Guinard
,
François Lanza
,
Christian Gachet
,
Catherine Léon
,
Anita Eckly
1
Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS
Chapters
00:04
Introduction
01:22
Preparation of the Experimental Set Up
01:51
Bone Marrow Sectioning and Placement into the Incubation Chamber
03:13
Real-Time Observation of Marrow Explants and Quantification of the Proplatelet-Extending Megakaryocytes
04:02
Results: Morphological Assessment of Megakaryocytes in Explants
04:45
Conclusion
Summary
Automatic Translation
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在这里,我们详细介绍了骨髓外植体方法,从样品制备到显微载玻片分析,以评估在其生理环境中分化的巨核细胞形成丙酸盐的能力。
Tags
Proplatelet Formation
Mouse Bone Marrow Explants
Megakaryocytes
Real-time Analysis
Thrombophilic Disease
Tyrode’s Buffer
Incubation Chamber
Time-lapse Microscopy
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