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ヒト多能性幹細胞からの3Dレチナル組織の導出のためのレチナルオガノイド誘導システム
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JoVE Journal
Medicine
Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
ヒト多能性幹細胞からの3Dレチナル組織の導出のためのレチナルオガノイド誘導システム
DOI:
10.3791/62435-v
•
10:05 min
•
April 12, 2021
•
Yuanyuan Guan*
1
,
Bingbing Xie*
1
,
Xiufeng Zhong
1
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center
,
Sun Yat-sen University, Guangzhou, China, 510060
Chapters
00:04
Introduction
01:19
Human Pluripotent Stem Cells (hPSCs) Culture
03:48
Embryoid Body (EB) Formation
05:56
Seed the Embryoid Bodies (EBs)
07:00
Detach and Culture OVs and RPE in Suspension to Obtain Retinal Organoids (ROs)
07:45
Results: Analysis of Morphological Features of ROs and Subtypes of Retinal Cells from hPSCs
09:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、ヒトの多能性幹細胞株が再現性と効率の高い人工組織を生成するのに適した最適化されたレチナルオルガノイド誘導システムについて説明します。
Tags
Retinal Organoid
Human Pluripotent Stem Cells
Retinal Induction
Retinal Development
Disease Modeling
Cell Therapy
Retinal Degenerative Diseases
Embryoid Body
Plating Density
Retinal Cells
EDTA Dissociation
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