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Journal
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Immunology and Infection
/
伪多莫纳斯航空
感染模型实时评估工具
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Tools for the Real-Time Assessment of a
Pseudomonas aeruginosa
Infection Model
Please note that all translations are automatically generated.
Click here for the English version.
伪多莫纳斯航空
感染模型实时评估工具
DOI:
10.3791/62420-v
•
07:39 min
•
April 06, 2021
•
Alexa D. Gannon
,
Sophie E. Darch
1
Department of Molecular Medicine
,
Morsani College of Medicine, University of South Florida
Chapters
00:05
Introduction
00:34
Synthetic Cystic Fibrosis Medium (SCFM2) Preparation
01:03
Bacterial Cell Suspension Preparation
02:36
Pseudomonas aeruginosa
Aggregate Visualization and Isolation
04:48
Image Analysis
06:15
Results: Analysis of
Pseudomonas aeruginosa
Aggregates
07:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
合成囊性纤维化痰介质(SCFM2)可与共聚焦激光扫描显微镜和荧光活性细胞分拣相结合,以高分辨率观察细菌聚集物。本文详细介绍了在抗菌治疗期间评估总人口的方法,作为未来研究的平台。
Tags
Pseudomonas Aeruginosa
Infection Model
Real-time Assessment
Antibiotic Resistance
Mucin
Synthetic Cystic Fibrosis Sputum Medium
Confocal Laser Scanning Microscope
Bacterial Aggregates
Optical Density
Log Phase
PBS Wash
Static Incubation
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