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用于低肿瘤含量组织中肿瘤富集的自动解剖方案

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Cancer Research

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JoVE Journal Cancer Research

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues

Please note that all translations are automatically generated. Click here for the English version.

用于低肿瘤含量组织中肿瘤富集的自动解剖方案

DOI:

10.3791/62394-v

•

06:44 min

•

March 29, 2021

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Charles A. Havnar, Oliver Zill, Jeff Eastham, Jeffrey Hung, Manana Javey, Emmanuel Naouri, Jennifer Giltnane, Justin M. Balko, Andrew Wallace, Nicolas Lounsbury, Daniel Oreper, Sarajane Saturnio, G-Y Yang, Amy A. Lo

¹Departments of Research Pathology,Genentech, ²Bioinformatics & Computational Biology,Genentech, ³Roche Sequencing Solutions, Hacienda Drive, ⁴Department of Medicine,Vanderbilt University Medical Center, Medical Center Drive, ⁵Department of Pathology,Northwestern University, Feinberg School of Medicine

Chapters

00:05Introduction
00:43Automated Tissue Dissection
05:23Results: Confirmation of the Automated Dissection
06:19Conclusion

Summary

Automatic Translation

具有自动组织解剖的数字注释为低肿瘤含量病例中的肿瘤富集提供了一种创新方法，并且适用于石蜡和冷冻组织类型。所描述的工作流程提高了准确性，可重复性和通量，可应用于研究和临床环境。

Tags

Automated Dissection Tumor Enrichment FFPE Macro Dissection Laser Capture Micro Dissection Digital Reference Image Stage Reference Image Tumor Regions Of Interest Milling Job Section Thickness Paraffinized Deparaffinized Scan Stage Transform Tool

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