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用于SARS-CoV-2检测和定量的两步逆转录液滴数字PCR方案
JoVE Journal
Biology
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JoVE Journal Biology
Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
DOI:

07:54 min

March 31, 2021

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Chapters

  • 00:04Introduction
  • 00:48cDNA and Droplet Digital Polymerase Chain Reaction (ddPCR) Preparation
  • 02:03Automated Droplet Generation
  • 02:59PCR Amplification and Droplet Reading
  • 04:09Data Analysis
  • 06:01Results: SARS‐CoV‐2 Detection Using a Two‐Color ddPCR System
  • 07:07Conclusion

Summary

Automatic Translation

这项工作总结了使用双色ddPCR系统开发SARS-CoV-2检测的不同检测方法的步骤。这些步骤很详细,并且已经包括有关如何改进测定和实验性能的说明。这些检测可用于多种SARS-CoV-2 RT-ddPCR应用。

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