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Biology
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多参数灌注对照分离原代大鼠肝细胞
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control
Please note that all translations are automatically generated.
Click here for the English version.
多参数灌注对照分离原代大鼠肝细胞
DOI:
10.3791/62289-v
•
07:35 min
•
April 05, 2021
•
Inn Chuan Ng*
1
,
Li Zhang*
2
,
Narelle Nichola Yi Ying Shen
,
Yun Ting Soong
,
Chan Way Ng
,
Phoebe Kang Sheing Koh
,
Yan Zhou
,
Hanry Yu
3,4,5,6
1
Department of Physiology & The Institute for Digital Medicine (WisDM)
,
National University of Singapore
,
2
College of Agriculture and Biology
,
Zhongkai University of Agriculture and Engineering
,
3
Mechanobiology Institute
,
National University of Singapore
,
4
Institute of Bioengineering and Nanotechnology
,
A*STAR
,
5
NUS Graduate School for Integrative Sciences and Engineering
,
6
CAMP
,
Singapore-MIT Alliance for Research and Technology
Chapters
00:04
Introduction
00:45
Portal Vein Cannulation
03:54
Liver Perfusion, Digestion, Hepatocyte Isolation
05:30
Results: Estimation of Purity and Function of Isolated Hepatocytes
07:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议详细说明了使用特殊的静脉内导管,标准化的无菌一次性导管,通过实时监测补充的温度控制,以及用于两步胶原酶灌注手术的报警系统,以提高分离的原代大鼠肝细胞的活力,产量和功能的一致性。
Tags
Primary Rat Hepatocytes
Perfusion Control
Liver Resection
Collagenase Recirculation
Portal Vein Cannulation
Inferior Vena Cava
Flow Rate
Hepatocyte Isolation
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