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Biology
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極低温保存細胞からの微視的データ収集
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
Please note that all translations are automatically generated.
Click here for the English version.
極低温保存細胞からの微視的データ収集
DOI:
10.3791/62274-v
•
11:55 min
•
May 28, 2021
•
Nina Vyas*
1
,
Nina Perry*
1
,
Chidinma A. Okolo
,
Ilias Kounatidis
,
Thomas M. Fish
,
Kamal L. Nahas
2
,
Archana Jadhav
,
Mohamed A. Koronfel
,
Johannes Groen
,
Eva Pereiro
,
Ian M. Dobbie
,
Maria Harkiolaki
1
Harwell Science and Innovation Campus
,
Beamline B24, Diamond Light Source
,
2
Division of Virology, Department of Pathology
,
University of Cambridge
,
3
ALBA Synchrotron
,
Beamline 09 – MISTRAL
,
4
Micron Advanced Imaging Consortium, Department of Biochemistry
,
University of Oxford
Chapters
00:04
Introduction
01:09
Preparation of the Cryo-Stage
02:08
Transfer of the Sample Storage Box into the Cryo-Stage
03:34
Stage Docking and Focusing
05:03
Brightfield Mosaic Acquisition and Identification of Areas of Interest
06:48
Data Collection
08:59
After Imaging
09:38
Results: Cryo-structured Illumination Microscopic Imaging
11:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、クライオ構造の照明顕微鏡を用いて、生物学的に凍結保存されたサンプルを画像化する方法を示す。U2OS細胞の細胞骨格をイメージングして方法論を実証する。
Tags
Cryo-structured Illumination Microscopy
Super Resolution Cryo Imaging
Cellular Ultrastructure
Vaccine Production
Antibody Engineering
Nano Particle Characterization
Cryo-stage
Liquid Nitrogen
Sample Transfer Cassette
TEM Grid
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